Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 680

货号：A27042

规格：1mg

价格：3310

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 680

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Recombinant full-length protein	用法：	0.05-2μg/mL(WB)

The sensitivity and specificity of each lot is confirmed using Western blot.Minimal cross-reactivity with rabbit, mouse, rat, human, bovine, guinea pig and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.

数据

Rabbit IgG (H+L) Secondary Antibody (A27042) in WBExpression of endogenous IRAK1 (~80KDa) was detected using the Rabbit anti- IRAK1 ABfinityTM primary antibody (0.5 µg/mL, 710015) and Goat anti-Rabbit IgG (H+L) SuperclonalTM Secondary Antibody, Alexa Fluor 680 Conjugate (Product # A27042) (0.4 µg/mL final concentration, 1:2500 dilution). Western blot analysis was performed on whole cell extracts (20 µg lysate) of various cell types using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System.

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