| Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21074) in IFImmunofluorescence analysis of Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 660 (Product # A-21074) was performed using A-431 cells stained with EGFR (EP38Y) Rabbit Monoclonal Antibody (Product # MA5-14485). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL of rabbit primary antibody for 3 hours at room temperature. Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 660 (Product # A-21074) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of EGFR in the membrane (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. |
| 1. Nature medicineSAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia."A21074 was used in flow cytometry to demonstrate that SAMHD1 reduces nucleoside analog cytarabine cytotoxicity in acute myeloid leukemia cells"AuthorsSchneider C,Oellerich T,Baldauf HM,Schwarz SM,Thomas D,Flick R,Bohnenberger H,Kaderali L,Stegmann L,Cremer A,Martin M,Lohmeyer J,Michaelis M,Hornung V,Schliemann C,Berdel WE,Hartmann W,Wardelmann E,Comoglio F,Hansmann ML,Yakunin AF,Geisslinger G,Ströbel P,Ferreirós N,Serve H,Keppler OT,Cinatl J2. Journal of leukocyte biologyCytolytic cells induce HMGB1 release from melanoma cell lines.AuthorsIto N,DeMarco RA,Mailliard RB,Han J,Rabinowich H,Kalinski P,Stolz DB,Zeh HJ,Lotze MT3. The Journal of biological chemistryEvidence for a granule targeting sequence within platelet factor 4.AuthorsEl Golli N,Issertial O,Rosa JP,Briquet-Laugier V4. The Journal of biological chemistryTyrosine phosphorylation of VE-cadherin prevents binding of p120- and beta-catenin and maintains the cellular mesenchymal state.AuthorsPotter MD,Barbero S,Cheresh DA |
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