Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 647
货号：A28181
规格：1mg
价格：3337
产品类型：荧光二抗
品牌：Thermo Fisher
物种：小鼠
宿主：山羊
抗体亚型：IgG
荧光染料：Alexa Fluor 647
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Recombinant full-length protein | 用法： | 0.1-1 μg/mL (ICC)；0.1-1 μg/mL (IF) |
The sensitivity and specificity of each lot is confirmed using Immunocytochemistry.Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig, and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
数据 |
| Mouse IgG (H+L) Secondary Antibody (A28181) in IFTime course showing induction of TNF-a signaling cascade upon treatment: Cellular localization of proteins in the NF-KB signaling pathway was detected upon treatment of HeLa cells with TNF-a (50 ng/mL) for 5, 10 and 25 min, respectively. Fixed and permeabilized cells were stained with ABfinity™ anti-IKK alpha/beta (pSpS176/180) Recombinant Rabbit Monoclonal Antibody (Product # 701643, 1 µg/mL) or anti-NF-kB Mouse Monoclonal Antibody (Product # 33-9900, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 647 conjugate (Product # A28181, 0.4 µg/mL, 1:2500). Images show staining of phospho-IKK alpha/beta and NF-KB (a, e, i, m) in untreated cells. No significant basal levels of phosphorylated IKK alpha/beta (a; green) were detected. Treatment with TNF alpha led to an increase in the levels of phospho-IKK alpha/beta (b - d ; green) in the cytosol and the nucleus, and a corresponding translocation of NF-kB to the nucleus (f - h; red). The composite images are shown in i - l; green, red. Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50) and m - p; green, red represent the specific localization of the proteins with reference to DAPI. No background staining was observed in control cells with no primary antibody (q). |
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参考文献： |
| 1.Neurobiology of disease In vivocharacterization of the aspartyl-tRNA synthetase DARS: Homing in on the leukodystrophy HBSL. "A28181 was used in immunohistochemistry - frozen section to study DARS expression in mice and create a mouse model of hypomyelination with brain stem and spinal cord involvement and leg spasticity" AuthorsFröhlich D,Suchowerska AK,Spencer ZH,von Jonquieres G,Klugmann CB,Bongers A,Delerue F,Stefen H,Ittner LM,Fath T,Housley GD,Klugmann M |
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