Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647/鸡抗兔 IgG (H+L) 交叉吸附，Alexa Fluor 647

货号：A-21443

规格：500μl

价格：3563

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：其它

抗体亚型：IgG

荧光染料：Alexa Fluor 647

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（IHC）；4 μg/mL (ICC)；4 μg/mL (IF)

This chicken anti-rabbit antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.Fluorescence of this long-wavelength Alexa Fluor dye is not visible by looking through a conventional fluorescence microscope.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21443) in IFImmunofluorescence analysis of Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor® 647 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA516891) The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor® 647 conjugate (Product # A-21443) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Journal of visualized experiments : JoVEQuantitative 3D In Silico Modeling (q3DISM) of Cerebral Amyloid-beta Phagocytosis in Rodent Models of Alzheimer's Disease."A21443 was used in immunohistochemistry - paraffin section to design a quantitative 3D in silico modeling platform allowing for true 3D quantitation of amyloid-beta phagocytosis by mononuclear phagocytes in rodent Alzheimer's Disease models"AuthorsGuillot-Sestier MV,Weitz TM,Town T2. Journal of cutaneous pathologyLoss of primary cilia correlates with cytologic severity in dysplastic melanocytic nevi."A21443 was used in immunohistochemistry - paraffin section to investigate cilia in cases of dysplastic nevi"AuthorsLang UE,Cheung C,Vladar EK,Swetter SM,Kim J3. Breast cancer research : BCRImaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods."A-21443 was used in immunohistochemistry - paraffin section to study the architecture of the mouse mammary gland using high-resolution 3D imaging"AuthorsLloyd-Lewis B,Davis FM,Harris OB,Hitchcock JR,Lourenco FC,Pasche M,Watson CJ4. Nature neuroscienceAge-related myelin degradation burdens the clearance function of microglia during aging."A21443 was used in immunohistochemistry to characterize age-related myelin fragmentation"AuthorsSafaiyan S,Kannaiyan N,Snaidero N,Brioschi S,Biber K,Yona S,Edinger AL,Jung S,Rossner MJ,Simons M5. Development (Cambridge, England)Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development."A21443 was used in immunohistochemistry to report that SDC4 governs flow-induced lymphatic endothelial cell polarization via regulation of VANGL2 expression"AuthorsWang Y,Baeyens N,Corti F,Tanaka K,Fang JS,Zhang J,Jin Y,Coon B,Hirschi KK,Schwartz MA,Simons M

技术参数

产品应用 ICC;IF;IHC

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