F(ab')2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555

货号：A-21430

规格：250μl

价格：2787

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 555

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	4 μg/mL (ICC)；4 μg/mL (IF)

Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21430) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 (Product # A-21430) was performed using HepG2 cells stained with alpha-1 antitrypsin Rabbit Polyclonal Antibody (Product # PA5-16661). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with rabbit primary antibody (1:250 dilution) for 3 hours at room temperature. F (ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 555 was used at concentration of 4µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha-1 antitrypsin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Biochemical pharmacologyMetformin represses glucose starvation induced autophagic response in microvascular endothelial cells and promotes cell death."A21430 was used in immunocytochemistry to describe the effect of metformin on endoplasmic reticulum stress and autophagy in glucose-starved micro-vascular endothelial cells"AuthorsSamuel SM,Ghosh S,Majeed Y,Arunachalam G,Emara MM,Ding H,Triggle CR2. Methods in cell biologyVisualization of cleavage furrow proteins in fixed dividing spermatocytes."A-21430 was used in immunocytochemistry and proximity ligation assay to describe protocols to study male Drosophila meiotic cells"AuthorsFrappaolo A,Sechi S,Belloni G,Piergentili R,Giansanti MG3. Breast cancer (Dove Medical Press)The role of the chemokine receptor XCR1 in breast cancer cells."A21430 was used in immunocytochemistry to measure the expressions of XCR1 mRNA breast cancer cell lines"AuthorsYang XL,Qi LG,Lin FJ,Ou ZL4. Journal of cell scienceError-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin."A21430 was used in immunocytochemistry to study the function of unique mouse oocyte acentriolar microtubule-organizing centers in meiotic spindle formation"AuthorsBaumann C,Wang X,Yang L,Viveiros MM5. Nature communicationsR function and expression with rapid antidepressant properties."A-21430 was used in immunocytochemistry to investigate how acute ethanol exposure results in lasting antidepressant and anxiolytic behaviors"AuthorsWolfe SA,Workman ER,Heaney CF,Niere F,Namjoshi S,Cacheaux LP,Farris SP,Drew MR,Zemelman BV,Harris RA,Raab-Graham KF

技术参数

产品应用 ICC;IF

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