Attune™ 仪器性能测试微球用来和 Attune™ 聚焦细胞分析软件联合使用，用于 Attune™ 声波聚焦细胞分析仪性能的日常的自动鉴别、测试和报告。定期用这些微球校准仪器将确保仪器的高精确度和高灵敏度，并能轻易识别其性能潜在的变化趋势。性能测试微球由多种标记不同强度荧光的独立微球亚群组成，用于仪器的优化动态范围设置。这些微球已在工厂预先进行了校正，每批次微球对应的性能数据作为一个下载文件,用于 Attune™ 细胞分析软件。每小瓶足够做50次测试。

Achieve functional knockout in human primary T-cells

T cell receptor knockout using TrueGuide Synthetic gRNAs. Human T cells were isolated and activated using Dynabeads magnetic beads (Cat. Nos. 11344D and 11141D, respectively) and then transfected with TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA for T cell receptor alpha (TRAC) or beta (TRBC) regions using the Neon Transfection System. Following transfection, editing efficiency was measured by the GeneArt Cleavage Detection assay (a) or by measuring % T cell receptor negative (TCR–) cells using the Attune NxT Flow Cytometer (b). Cells analyzed by flow cytometry were stained with a T cell receptor–specific antibody conjugated to PE (c).