CD197 (CCR7) Monoclonal Antibody (3D12), PE

货号：12-1979-41,12-1979-42

规格：25 Tests,100 Tests

价格：1029,2476

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：3D12

荧光染料：PE

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE, eBioscience™
免疫原性：		用法：	5uL（0.125µg）/test(Flow）

Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DC's. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.It is recommended that the staining incubation time be increased to at least 45 minutes at 2-8°C for optimal staining.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

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参考文献：

1. Cancer biology and therapyDurable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy."12-1979 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy."AuthorsWyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ2. BioResearch open accessThe APPEESFRS Peptide, Restricted by the HLA-B*35:01 Molecule, and the APPEESFRF Variant Derived from an Autologous HIV-1 Strain Induces Polyfunctional Responses in CD8+ T Cells."12-1979 was used in Flow cytometry/Cell sorting to determine the effector profile and maturation phenotype of CD8+ T-cells targeting the consensus APPEESFRS (AS9) epitope and its variant APPEESFRF (AF9)."AuthorsAcevedo-Sáenz L,Carmona-Pérez L,Velilla-Hernández PA,Delgado JC,Rugeles L MT3. The Journal of pediatricsCalcineurin inhibitor treatment of intravenous immunoglobulin-resistant Kawasaki disease."12-1979 was used in Flow cytometry/Cell sorting to investigate treatment of refractory KD with a calcineurin inhibitor."AuthorsTremoulet AH,Pancoast P,Franco A,Bujold M,Shimizu C,Onouchi Y,Tamamoto A,Erdem G,Dodd D,Burns JC4. Journal of virologyHuman CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection."12-1979 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection."AuthorsKotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A5. British journal of haematologyRole of TLX1 in T-cell acute lymphoblastic leukaemia pathogenesis.

技术参数

产品应用 Flow

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