FxCycle™ Violet stain (F10347) is used for flow cytometric analysis of DNA content in fixed cells. This dye is excited with a 405⁄407 nm laser with emission collected in the 450⁄50 BP filter or equilavent. This dye is DNA selective so RNAse is not required. With multicolor cell cycle studies possible using flow cytometry, it is often necessary to analyze DNA content on alternative lasers to preserve the common 488 nm laser and detection channels for other markers. Well suited for the popular violet laser line, FxCycle™ Violet stain is a good choice for DNA content analysis in multicolor cell cycle studies.
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