DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight 550/FLAG标签抗体

货号：MA1-91878-D550

规格：50 µL

价格：2875

产品类型：标签抗体

品牌：Thermo Fisher

物种：恒河猴/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG

荧光染料：其它

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	Mouse / IgG2b
免疫原性：	Synthetic peptide (DYKDDDDK) coupled to KLH.	用法：	5µg/mL (ICC);5µg/mL（IF)

Product Specific InformationMA1-91878-D550 has been successfully used for immunofluorescence. DyLight 550 has an excitation/emission of 562/576 nm.similar epitope as the FLAG tag from SigmaBackground/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

DYKDDDDK Tag Antibody (MA1-91878-D550) in IFImmunofluorescence analysis of FLAG™ Epitope Tag (DYKDDDDK) was performed using 70% confluent log phase HeLa cells transfected with DYKDDDDK-BNIP3 construct. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with DYKDDDDK Tag Mouse Monoclonal Antibody (FG4R), DyLight 550 (Product # MA1-91878-D550) at 5 µg/mL in 0.1% BSA for overnight at 4°C (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: green) was stained with Alexa Fluor® 488 Phalloidin(Product # A12379, 1:100). Panel d represents the merged image showing cytoplasmic localization of BNIP3. Panel e shows the untransfected cells. Panel f shows the no primary control. The images were captured at 60X magnification. DYKDDDDK Tag Antibody (MA1-91878-D550) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a FLAG Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 550-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-D550) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

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参考文献：

1.Genome research U2AF1 mutations alter splice site recognition in hematological malignancies. View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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