6x-His Tag Monoclonal Antibody (4E3D10H2/E3), Alexa Fluor 647/His标签抗体
货号：MA1-135-A647
规格：50 µl
价格：3849
产品类型：标签抗体
品牌：Thermo Fisher
物种：Tag
宿主：小鼠
抗体亚型：其它
荧光染料：Alexa Fluor 647
点击Invitrogen标签抗体集，查看更多标签抗体
抗体类型: | 单抗 | 同型对照： | IgG1 |
免疫原性： | Mixture of N-terminal and C-terminal 6x-His peptides, CG-HHHHHH and HHHHHH-GC | 用法： | 1:25-1:100 (ICC);1:25-1:100（IF) |
Background/Target InformationThe 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| 6x-His Tag Antibody (MA1-135-A647) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a HIS Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 647 conjugated HIS-tag monoclonal antibody (Product # MA1-135-A647) at a dilution of 1:50 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. |
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