HA Tag Monoclonal Antibody (2-2.2.14), DyLight 680/HA标签抗体

货号：26183-D680

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 680

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	HA peptide YPYDVPDYA derivitized to ovalbumin.	用法：	1:25-1:100（ICC）;1:25-1:100（IF）;1:500-1:1000（WB)

Product Specific Information26183-D680 has been successfully used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.Background/Target InformationHA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HA Tag Antibody (26183-D680) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing an HA Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated HA Epitope Tag monoclonal antibody (Product # 26183-D680) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification. HA Tag Antibody (26183-D680) in WB Western blot analysis of HA Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated HA Epitope Tag monoclonal antibody (Product # 26183-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.

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参考文献：

1.PloS one Valproic acid causes proteasomal degradation of DICER and influences miRNA expression. "26183-D680 was used in western blot to study the proteasomal degradation of DICER and altered miRNA levels induced by valproic acid" AuthorsZhang Z,Convertini P,Shen M,Xu X,Lemoine F,de la Grange P,Andres DA,Stamm S

技术参数

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