Myc Tag Monoclonal Antibody (Myc.A7), HRP/Myc 标签抗体

货号：MA1-21316-HRP

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：HRP

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	Synthetic peptide: EQKLISEEDL (Myc).	用法：	1:500-1:2000（WB)

Product Specific InformationMA1-21316-HRP has been successfully used in Western blotting applications on myc tagged samples.Background/Target InformationEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Myc Tag Antibody (MA1-21316-HRP) in WBWestern blot analysis of Myc was performed by loading 50 µg of NCCIT and Jurkat lysates and 10 µg of Myc-MYCBP-containing cell lysate onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Myc Epitope Tag antibody (Product # MA1-21316-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075) at an exposure time of 2 minutes (NCCIT and Jurkat) and 3 seconds (Myc-MYCBP). Results show a band at ~57 kDa (NCCIT and Jurkat) and 16 kDa (Myc-MYCBP) as well as a 2 unknown bands at 35-40 kDa.

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参考文献：

1.Genetics DNA Replication Stress Phosphoproteome Profiles Reveal Novel Functional Phosphorylation Sites on Xrs2 in Saccharomyces cerevisiae. View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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