FITC Monoclonal Antibody (#9)/FITC抗体

货号：MA5-14696

规格：100 µg

价格：5441

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, lambda
免疫原性：	FITC-BSA	用法：	1:1000(WB)

Product Specific InformationProduct MA514696 is a smaller package size of MIF2902 (formerly sold as a Seradyn product).Background/Target InformationFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques sincethe conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

FITC Antibody (MA5-14696) in ELISADirect ELISA analysis of FITC was performed by coating wells of a plate with FITC-BSA or BSA at a concentration of 1 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer (Product # N503) and blocked with 300 µL of StartingBlock (PBS) Blocking Buffer (Product # 37538) for at least 1 hour at room temperature. After washing, 100 µL of FITC monoclonal antibody (Product # MA5-14696) was added to wells in duplicate at 1000, 500, 250, 125, 62, 31, 15, 7.8, 3.9, 1.9 and 0.9 ng/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100 µL per well of 1-Step Ultra TMB substrate (Product # 34028) per well for 5 minutes at room temperature in the dark. The reaction was stopped with 100 µL per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm. FITC Antibody (MA5-14696) in WBWestern blot analysis of Fluorescein Isothiocyanate (FITC) was performed by loading various amounts of a FITC-BSA conjugate and 10 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% milk in TBST for at least 1 hour at room temperature. FITC-BSA was detected at ~72 kD using a FITC monoclonal antibody (Product # MA5-14696) at a dilution of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:50,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).

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技术参数

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