GAPDH Loading Control Monoclonal Antibody (GA1R), DyLight 650/GAPDH抗体

货号： MA5-15738-D650

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：DyLight 650

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类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant GAPDH	用法：	1:25-1:100(ICC);1:25-1:100(IF

Product Specific InformationMA5-15738-D650 has successfully been used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672nm.Background/Target InformationGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GAPDH Loading Control Antibody (MA5-15738-D650) in IFImmunofluorescent analysis of GAPDH (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 650-conjugated GAPDH monoclonal antibody (Product # MA5-15738-D650) at a dilution of 1:50 for at least 1 hour at room temperature. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

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参考文献：

1.Journal of virologyProtein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.Nucleic acids researchThe amino-terminal tails of histones H2A and H3 coordinate efficient base excision repair, DNA damage signaling and postreplication repair in Saccharomyces cerevisiae.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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