beta Actin Monoclonal Antibody (15G5A11/E2)

货号：MA1-140

规格：100 ug

价格：3666

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

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类型:	单抗	同型对照：	IgG1
免疫原性：	Purified full-length recombinant human beta-actin expressed in E. coli	用法：	1:1000-1:4000(ICC);1:1000-1:4000(IF);2 µg(IP);1:1000-20000(WB)

Product Specific InformationBy Western blot, MA1-140 detects a prominent ~42kD protein in human, mouse, non-human primate, and rat whole cell lysates.For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.Background/Target InformationBeta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Actin Antibody (MA1-140) in WBWestern blot analysis of beta-actin was performed by loading 25 µg of various cell lysates and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543). The membrane was probed with a beta-actin monoclonal antibody (Product # MA1-140) at a dilution of 1:16,000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:40,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). NOTE: Similar results are obtained when blocking with 5% BSA in TBST. beta Actin Antibody (MA1-140) in IFImmunofluorescent analysis of beta-actin (green) in HeLa cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for 15 minutes at room temperature. Cells were probed with a beta-actin monoclonal antibody (Product # MA1-140) at a dilution of 1:2000, or incubated in blocking buffer as a negative control, overnight at 4°C, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:1000 for 30 minutes at room temperature. Nuclei (blue) were stained with DAPI (Product # 62248). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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参考文献：

1.European journal of medical researchProteomic analysis of exosomes derived from human lymphoma cells."MA1-140 was used in western blot to characterize the proteomic content of lymphoma cell-derived exosomes."AuthorsYao Y,Wei W,Sun J,Chen L,Deng X,Ma L,Hao S

技术参数

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