Aconitase 2 Monoclonal Antibody (7G4)

货号：MA1-029,MA1-029X

规格：100 ug,20 uL

价格：4809,2148

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	单抗	同型对照：	IgG1
免疫原性：	Full length human recombinant protein of human ACO2 (NP_001089) produced in HEK293T cell.	用法：	1:100(IF);1:100(ICC);1:800(IHC);1:1000(WB)

Target InformationThe protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Aconitase 2 Antibody (MA1-029) in IFImmunofluorescent analysis of Aco-2 using anti-Aco-2 monoclonal antibody (Product # MA1-029) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing Aco-2 (Product # MA1-029), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35503) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. Aconitase 2 Antibody (MA1-029) in WBWestern blot analysis of Aco-2 was performed by loading 25 µg of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing Aco-2 (Product # MA1-029) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1% Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product # 34075).

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