7-AAD (7-Aminoactinomycin D) 7AAD

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7-AAD (7-Aminoactinomycin D) 7AAD

货号:A1310

规格:1 mg

价格:2459

产品类型:细胞凋亡检测

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies.
数据:

Wheat root tips in seven stages of the cell cycle. 7-aminoactinomycin D.Panel of confocal micrographs showing cells from wheat root tips in seven stages of the cell cycle. DNA was stained with 7-aminoactinomycin D (A1310]), and microtubules were labeled with an anti&endash;ß-tubulin antibody in conjunction with a fluorescein-labeled secondary antibody. Cells vary in width from about 15 µm to about 25 µm. The stages are (from top left): interphase cortical microtubule array; pre-prophase band of microtubules (predicts future plane of division); metaphase mitotic spindle; telophase, showing early phragmoplast and cell plate; fully developed phragmoplast during cytokinesis; late cytokinesis (plane of division matching plane of earlier pre-prophase band); restoration of cortical arrays in daughter cells. Image contributed by B.E.S. Gunning, Plant Cell Biology Group, Research School of Biological Sciences, Australian National University. Used with permission from Gunning, B.E.S. and Steer, M.W., Plant Cell Biology: Structure and Function, Jones and Bartlett Publishers (1995).
相关产品:
▪细胞周期检测▪细胞凋亡检测▪细胞增殖活性检测
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