NPM1 Monoclonal Antibody (FC-61991)

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NPM1 Monoclonal Antibody (FC-61991)

货号:32-5200

规格:100 µg

价格:4809

产品类型:流式抗体

品牌:Thermo Fisher

物种:人/小鼠/大鼠

宿主:小鼠

抗体亚型:其它

荧光染料:其它

类型:

单抗

同型对照:

IgG1

免疫原性:

Extracts from chicken nuclei

用法:

1-2 µg/mL(ELISA);2-10 µg/mL(IF);2-10 µg/mL(ICC);1-3 µg/mL(WB);10 µg(IP)

Product Specific InformationThis antibody reacts with the C-terminus of Nucleophosmin (B23). Positive controls used: HeLa, K562, Jurkat, MCF-7, and HL60 cell lysates). In western blots the antibody recognizes a band at ~37 kDa.Target InformationNPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

NPM1 Antibody (32-5200) in IHCImmunohistochemical staining of human colon cancer tissue using Mouse anti-Nucleophosmin monoclonal antibody (clone FC-61991) (Product # 32-5200).

NPM1 Antibody (32-5200) in IF

Immunofluorescence analysis of Nucleophosmin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nucleophosmin (FC-61991) Mouse Monoclonal Antibody (Product # 32-5200) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conj µgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleolar localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

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参考文献:

1.BMC biochemistryTIPT2 and geminin interact with basal transcription factors to synergize in transcriptional regulation."32-5200 was used in immunohistochemistry to review the transcription factors that the re-replication inhibitor Geminin binds"AuthorsPitulescu ME,Teichmann M,Luo L,Kessel M2.BloodImmunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia."32-5200 was used in immunohistochemistry to assess the role of nucleophosmin exon-12 mutations in adult acute myeloid leukemia"AuthorsFalini B,Martelli MP,Bolli N,Bonasso R,Ghia E,Pallotta MT,Diverio D,Nicoletti I,Pacini R,Tabarrini A,Galletti BV,Mannucci R,Roti G,Rosati R,Specchia G,Liso A,Tiacci E,Alcalay M,Luzi L,Volorio S,Bernard L,Guarini A,Amadori S,Mandelli F,Pane F,Lo-Coco F,Saglio G,Pelicci PG,Martelli MF,Mecucci C3.The Journal of clinical investigationNuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy."32-5200 was used in Immunofluorescence to study the effect of overexpressing nuclear pore protein NUP88 on the probability of intestinal tumors in mice."AuthorsNaylor RM,Jeganathan KB,Cao X,van Deursen JM4.NatureXRCC1 mutation is associated with PARP1 hyperactivation and cerebellar ataxia."32-5200 was used in western blot to investigate the role of XRCC1 protein complexes in normal neurological function and identify PARP1 as a therapeutic target in DNA strand break repair-defective disease"AuthorsHoch NC,Hanzlikova H,Rulten SL,Tétreault M,Komulainen E,Ju L,Hornyak P,Zeng Z,Gittens W,Rey SA,Staras K,Mancini GM,McKinnon PJ,Wang ZQ,Wagner JD,Yoon G,Caldecott KW5.Biochemical and biophysical research communicationsFunctional characterization and efficient detection of Nucleophosmin/NPM1 oligomers."325200 was used in western blot to examine the functional meaning of oligomerization of NPM1/nucleophosmin"AuthorsLin J,Hisaoka M,Nagata K,Okuwaki M

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