HDAC2 Polyclonal Antibody

货号：PA1-861

规格：100 µg

价格：5468

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2.	用法：	1-3 µL（CHIP）；1-20 µg/mL（Flow）;1.0 µg/mL(ICC);1:50 - 1:200(IF);0.4 µg/mL(IHC);0.5 µg/mL(WB)

Product Specific InformationPA1-861 detects histone deacetylase 2 (HDAC2) from canine, hamster, human, mouse, non-human primate, rat tissues and cells.PA1-861 has been successfully used in Western blot, ChIP, immunoprecipitation, immunofluorescence, flow cytometry, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~55 kDa protein representing HDAC2 and a ~40 kDa protein of unknown identity from HeLa cell lysate. Immunocytochemical staining of HDAC2 in HeLa cells with PA1-861 results in nuclear staining.The PA1-861 immunogen is a synthetic peptide corresponding to residues C T(475) D T K G T K S E Q L S N P(488) of human HDAC2. This immunizing peptide (Cat. # PEP-070) is available for use in neutralization and control experiments.Target InformationHistone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly concerved from yeast to human.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

HDAC2 Antibody (PA1-861) in IFImmunofluorescent analysis of HDAC2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification. HDAC2 Antibody (PA1-861) in IFImmunofluorescent analysis of HDAC2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552). HDAC2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

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参考文献：

1.International journal of oncologyHistone deacetylases meet microRNA-associated MMP-9 expression regulation in glucocorticoid-sensitive and -resistant cell lines."PA1861 was used in western blot to suggest that the use of HDAC inhibitors in a context of acquired glucocorticoid resistance may modify the epigenetic landscape"AuthorsHentati-Kallel M,Le Jan S,Bernard P,Antonicelli F,Trussardi-Régnier A2.The Journal of biological chemistryProtein kinase CK2 regulates the dimerization of histone deacetylase 1 (HDAC1) and HDAC2 during mitosis."PA1-861 was used in western blot to study the modulation of the dimerization of HDAC-1 and -2 by CK2-mediated phosphorylation during mitosis"AuthorsKhan DH,He S,Yu J,Winter S,Cao W,Seiser C,Davie JR3.The Journal of biological chemistryProteomic and functional analyses reveal the role of chromatin reader SFMBT1 in regulating epigenetic silencing and the myogenic gene program."PA1-861 was used in western blot to study the mechanism by which SFMBT1 modulates epigenetic silencing and mitogenic gene expression"AuthorsLin S,Shen H,Li JL,Tang S,Gu Y,Chen Z,Hu C,Rice JC,Lu J,Wu L4.The Journal of biological chemistryTranscriptional repression of peroxisome proliferator-activated receptor beta/delta in murine keratinocytes by CCAAT/enhancer-binding proteins."PA1-861 was used in western blot to study the relationship between PPARbeta and C/EBP transcription factors and its effect on cell proliferation and differentiation."AuthorsDi-Poï N,Desvergne B,Michalik L,Wahli W5.Nucleic acids researchModification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription."PA1-861 was used in western blot to study the regulation of DNA methyltransferase Dnmt3a enzymatic activity and transcriptional regulation."AuthorsLing Y,Sankpal UT,Robertson AK,McNally JG,Karpova T,Robertson KD

技术参数

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