alpha Tubulin Monoclonal Antibody (TU-01), Alexa Fluor 555

货号：MA1-38000-A555

规格：50 µl

价格：4223

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG

荧光染料：Alexa Fluor 555

类型:	单抗	同型对照：	IgG1
免疫原性：	Reacts with peptides that correspond to aa 65-79 in the N-terminus of alpha-tubulin	用法：	1-10 µg/mL(ICC);1-10 µg/mL(IF)

Target InformationAlpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

alpha Tubulin Antibody (MA1-38000-A555) in IFImmunofluorescent analysis of Alpha Tubulin (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Alpha Tubulin Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA1-38000-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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