SSEA1 Monoclonal Antibody (MC-480), DyLight 550
货号： MA1-022-D550
规格：100 µL
价格：4006
产品类型：流式抗体
品牌：Thermo Fisher
抗原：SSEA1
物种：人/小鼠
宿主：小鼠
抗体亚型：IgM
克隆号：MC-480
荧光染料：DyLight 550
类型: | 单抗 | 同型对照： | IgM |
免疫原性： | BALB/c mouse immunized with F9 teratocarcinoma stem cells (X-irradiated). | 用法： | 1:50-1:500(Flow) |
Product Specific InformationMA1-022-D550 has been successfully used in flow cytometry on mouse and human samples.Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| SSEA1 Antibody (MA1-022-D550) in FlowFlow cytometry analysis of SSEA-1 in F9 (red histogram) and NCCIT cells (orange histogram) compared to unstained cells (blue histogram). Positive staining is observed on F9 cells when compared to no antibody control (middle panel) and to SSEA-1-negative undifferentiated NCCIT cells (right panel). Cells were fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with 2% BSA-PBS for 30 minutes at room temperature and incubated with a DyLight 550-conjugated SSEA-1 monoclonal antibody (Product # MA1-022-D550) in 2% BSA-PBS at a dilution of 1:100 for 60 minutes at room temperature. Cells were washed and re-suspended in PBS for FACS analysis. |
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