SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience™

货号： 12-8813-42

规格：200 tests

价格：2987

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：eBioMC-480 (MC-480)

荧光染料：PE

类型:	单抗	同型对照：	IgM
免疫原性：		用法：	5 µL (0.125 µg)/test(Flow)

Product Specific InformationDescription: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of the F9 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA1 Antibody (12-8813-42) in FlowStaining of the F9 cell line with Mouse IgM Isotype Control PE (Product # 12-4752) (open histogram) or Anti-Human/Mouse SSEA-1 PE (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.NatureComparative glycoproteomics of stem cells identifies new players in ricin toxicity."12-8813 was used in Flow cytometry/Cell sorting to develop a comparative glycoproteomics platform for studying genome-wide protein glycosylation and glycan modifications in complex biological systems."AuthorsStadlmann J,Taubenschmid J,Wenzel D,Gattinger A,Dürnberger G,Dusberger F,Elling U,Mach L,Mechtler K,Penninger JM2.Cell cycle (Georgetown, Tex.)Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer."12-8813 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning."AuthorsQin Y,Qin J,Zhou C,Li J,Gao WQ3.Cell death and differentiationΔNp63 regulates select routes of reprogramming via multiple mechanisms."12-8813 was used in Flow cytometry/Cell sorting to report that in stark contrast to p53, p73 has no role in the molecular mechanisms of reprogramming the conversion of somatic cells into induced pluripotent stem cells."AuthorsAlexandrova EM,Petrenko O,Nemajerova A,Romano RA,Sinha S,Moll UM4.Development (Cambridge, England)Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos."12-8813 was used in Flow cytometry/Cell sorting to devise a method to make transparent whole mouse embryos for the observation of hematopoietic clusters."AuthorsYokomizo T,Dzierzak E5.Cell cycle (Georgetown, Tex.)Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer."12-8813 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning."AuthorsQin Y,Qin J,Zhou C,Li J,Gao WQ

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