QuickExtract™ DNA Extraction Solution 1.0, 50 mL/DNA提取试剂
货号：QE09050,QE0905T
规格：50mL,5 mL
价格：4991,938
产品类型：DNA提取
品牌：Lucigen
| QuickExtract™ DNA Extraction Solution provides a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification—all without the use of toxic chemicals or spin columns. DNA extraction requires only heat treatment to lyse the cellular or tissue material, release the DNA, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR. The procedure is easily scaled to process hundreds of samples in multiwell plates, using robotic automation systems. Many publications support the use of QuickExtract DNA Extraction Solution with samples such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells, zebrafish organs and scales, mouse tail snips, and more.The extracted DNA is suitable for PCR-based analysis, such as: genomic, transgenic, or viral DNA screening in animals; genetic or environmental research and screening in humans and other organisms; and CRISPR/Cas9 library screening.QuickExtract DNA Extraction Solution is provided at 5 mL and 50 mL volumes, sufficient for 10 or 100 extractions, respectively. QuickExtract™DNA提取解决方案提供了一种快速，简单且便宜的方法来制备用于PCR扩增的基因组DNA，而无需使用任何有毒化学物质或离心柱。 DNA提取仅需热处理即可裂解细胞或组织材料，释放DNA并降解抑制扩增的化合物。 热处理后，样品DNA即可用于PCR。 使用机器自动化系统，可轻松扩展该过程以在多孔板上处理数百个样品。 许多已发表文献支持将QuickExtract DNA Extraction Solution与样品一起使用，例如毛囊，羽毛的鹅毛末端细胞，组织培养细胞，颊细胞，斑马鱼的器官和鳞片，小鼠的尾巴等等。提取的DNA适用于基于PCR的分析，例如：动物的基因组，转基因或病毒DNA筛选； 人类和其他生物的遗传或环境研究和筛选； 和CRISPR / Cas9文库筛选。 提供5 mL和50 mL体积的QuickExtract DNA提取溶液，分别足以进行10或100次提取。 |
| 优点： |
| ▪ Fast: 8 minute extraction protocol for most sample types▪ Simple: No centrifugation steps of spin columns used to help increase yields▪ Automation-friendly: Simple protocol integrated easily into automated workflows▪ Safe: Uses only non-toxic reagents▪ 快速：8分钟提取方案，适用于大多数样品类型▪ 简单：无需使用离心柱离心步骤即可提高产量▪ 便利自动化：将简单操作轻松集成到自动化工作流程中▪ 安全：仅使用无毒试剂 |
| 数据： |
| Figure1. FailSafe™ PCR amplifications of genomic DNA isolated using the QuickExtract™ procedure. All samples were treated with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1–3, human β-globin(from human buccal cells, HeLa cells, and human hair follicle, respectively); lane 4, transgenic mouse GAPDH (from mouse tail snip); lane 5, 16S ribosomal RNA gene (from E. coli ); lane 6, transgenic SV40 T antigen (from mouse tail snip图1. 使用QuickExtract™操作对基因组DNA进行FailSafe™PCR扩增。 所有样品均用QuickExtract™DNA提取溶液处理。 使用引物进行PCR扩增所示区域：1-3道，人β-珠蛋白（分别来自人颊细胞，HeLa细胞和人毛囊）； 泳道4，转基因小鼠GAPDH（来自小鼠尾巴剪断）； 泳道5，16S核糖体RNA基因（来自大肠杆菌）； 泳道6，转基因SV40 T抗原（来自鼠尾巴） |
技术参数 产品优点 - Fast: 8 minute extraction protocol for most sample types
- Simple: No centrifugation steps of spin columns used to help increase yields
- Automation-friendly: Simple protocol integrated easily into automated workflows
- Safe: Uses only non-toxic reagents
- 快速：8分钟提取方案，适用于大多数样品类型
- 简单：无需使用离心柱离心步骤即可提高产量
- 便利自动化：将简单操作轻松集成到自动化工作流程中
- 安全：仅使用无毒试剂
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