CD43 Monoclonal Antibody (eBio84-3C1 (84-3C1)), eBioscience™

货号：14-0439-82

规格：100 µg

价格：1156

产品类型：流式抗体

品牌：eBioscience

抗原：CD43

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：eBio84-3C1 (84-3C1)

荧光染料：Purified

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	20 µg/mL(Flow)；20 µg/mL(IHC (P))

Product Specific InformationDescription: The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.Applications Reported: This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This eBio84-3C1 (84-3C1) antibody has been tested by flow cytometric analysis of human peripheral blood cells and by immunohistochemistry of formalin-fixed paraffin embedded human spleen using low pH antigen retreival at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD43 Antibody (14-0439-82) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human spleen stained with 20 µg/mL Anti-Human CD43 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase, and Fast Red. Nuclei are counterstained with hematoxylin.

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参考文献：

1.Stem cells translational medicineThe Generation of Human γδT Cell-Derived Induced Pluripotent Stem Cells from Whole Peripheral Blood Mononuclear Cell Culture.2.Biomedical reportsAberrant expression of interleukin-10 and activation-induced cytidine deaminase in B cells from patients with Behçet's disease.3.Stem cells (Dayton, Ohio)Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.4.Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.5.Arteriosclerosis, thrombosis, and vascular biologyProtective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance."14-0439 was used in Flow cytometry/Cell sorting to investigate the role of B-cell functions in obesity-induced metabolic dysfunction."AuthorsHarmon DB,Srikakulapu P,Kaplan JL,Oldham SN,McSkimming C,Garmey JC,Perry HM,Kirby JL,Prohaska TA,Gonen A,Hallowell P,Schirmer B,Tsimikas S,Taylor AM,Witztum JL,McNamara CA

技术参数

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