EconoTaq® DNA Polymerase (with Mg++)，Econotaq®DNA聚合酶（含Mg++）
货号：30031-1,30031-2,30031-3
规格：1000 U,5000 U (5 x 1000 U) ,10000 U (10 x 1000 U)
价格：1418,6818,13418
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| 该酶来自水生栖热菌（Thermus aquaticus）,在高于70℃的温度下具有最佳活性。它具有固定的5’→3’结构依赖性外切核酸酶活性，但缺3’→5’校正核酸外切酶活性。EconoTaq价格低而质量和性能却很高。随酶附赠一管含镁的缓冲液或一管单独的MgCl2溶液。 |
| 优点： |
-Great Taq Polymerase performance at a low price； -Choice of reaction buffers, with or without MgCl2； -Non-proofreading Polymerase. |
| 数据： |
| Figure 1.High purity of EconoTaq DNA Polymerase (SDS PAGE). Lane 1, broad range molecular weight markers; Lane 2, Lucigen EconoTaq DNA Polymerase.Figure 2.Taq DNA polymerase from Promega and New England Biolabs were compared to Lucigen’s EconoTaq DNA Polymerase (2 different lots) in amplifying the ampicillin gene (0.8 kb) in a pUC19 vector. (–), no DNA. (+), DNA added (40 ng). MW, 1 kb ladder.Figure 3.EconoTaq vs. AmpliTaq® (Applied Biosystems) DNA polymerase in genotyping. All PCR reactions were performed in a RoboCycler 96 (Stratagene). Hip1 genotyping was performed using the following PCR conditions: 94°C for 1min, 35 cycles of 94°C for 30sec, 62°C for 60sec, 72°C for 90sec, and 72°C for 7min. Shh, Cdo and Gas1 genotyping were performed using the following PCR conditions: 94°C for 2min, 35 cycles of 94°C for 60sec, 65°C for 60sec, 72°C for 90sec, and 72°C for 7min. All PCR reactions contained final concentrations of 1 µM of each primer, 200 µM dNTPs, 1X cresol red loading dye, and 1U of the indicated Taq polymerase. Sequences for all PCR primers have been previously published (references available). |
技术参数 产品优点 - Great Taq Polymerase performance at a low price.- Choice of reaction buffers, with or without MgCl2.- Non-proofreading Polymerase
产品应用 - DNA amplification - DNA模板的PCR和多重PCR扩增
产品类型 DNA聚合酶
组成成分 EconoTaq DNA Polymerase is provided with a choice of 10X Reaction Buffer with Mg++ (“with Mg++”); or 10X Reaction Buffer without Mg++ and a separate tube of 25 mM MgCl2 (“separate Mg++”).
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