LavaLAMP™ DNA Component Kit DNA组件试剂盒
货号：30076-1
规格：500 Reactions
价格：3444
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The LavaLAMP™ DNA Component Kit is intended to simplify development and optimization of DNA loopmediated isothermal amplification (LAMP) reactions. This kit is for research purposes only, and available under the limited-use license described at the end of this document. LAMP commonly employs a set of six primers (F3, B3, Loop-F, Loop-B, FIP, and BIP), which must be supplied by the user. Previously-established primer designs may be used. Alternatively, Lucigen recommends use of the free Eiken web utility or the commercially available LAMP Design Software from Premier BioSoft to design new primer sets (see Appendix A). Not all primer sets identified by these programs are guaranteed to work with LavaLAMP™ DNA Component Kit or any other LAMP system. We strongly encourage designing multiple primer sets to identify the best performing set. We also highly recommend inclusion of loop primers (Loop-F and Loop-B; Nagamine, 2002) to improve assay performance. LavaLAMP™ DNA amplification products may be detected by agarose gel electrophoresis or by realtime/end-point monitoring with fluorescent double-stranded DNA-binding dyes, such as the Lucigen Green Fluorescent Dye (Cat. No. 30078-1). Turbidity may also be monitored to assess amplification (Mori, 2001), but this method is less sensitive. |
| 优点： |
| ▪ Loop-mediated Isothermal Amplification (LAMP): Allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation▪ 68-74°C Reaction Temperature: Reduces background amplification and improves primer specificity depending on the target▪ Lyophilization-ready: Component formulations enable generation of room temperature stable test kits through lyophilization▪ Custom/Bulk Available: Custom volumes and dispensing available to match your specific requirements |
| 数据： |
| Figure1 : Positive LAMP Reaction Products from a Positive Control Target. Lane 1: 100 bp Marker, Lane 2: No Target Control reaction. Lanes 3 and 4: A distinct banding pattern is seen among the smear, which is indicative of a positive LAMP reaction. Figure 2: Typical Background Amplification in a LAMP Reaction. Lane 1: 100 bp ladder, Lanes 2 and 3: Non-specific or background amplification appears as a smear of DNA fragments with no visible or distinct bands. A prominent primer dimer band is also characteristic of non-specific amplification. Lane 4: Absence of non-specific amplification (no products). Fluorescent Signals from Different LAMP ReactionsFigure 3: Early Fluorescent Signals from Positive LAMP Reactions and Late Background Signals from NTC Controls after Extended Incubation. LAMP reactions were run in a real-time thermal cycler. The fluorescent signal from each reaction was captured over a 60-minute reaction time. The red and blue lines represent the fluorescent signals from Positive Control reactions with varying amounts of target. The black lines represent the non-specific background amplification signals that arise later in No Target Control reactions. |
| 组成成分： |
| ▪ 10X LavaLAMP™ DNA Buffer ：储存在-20°C▪LavaLAMP™ DNA Enzyme ：20μL/次；储存在-20°C▪Magnesium Sulfate, 100mM ：储存在-20°C▪DNA Positive Control LAMP Primer Mix ：储存在-20°C▪DNA Positive Control ：储存在-20°C |
| 相关产品： |
| ▪LavaLAMP™ DNA Component Kit with Dye（货号#30077-1）▪Green Fluorescent Dye（货号#30078） |
| 参考文献： |
| 1.Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000 28(12):E63. 2.Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002 16(3):223-9. |
技术参数 产品优点 - Loop-mediated Isothermal Amplification (LAMP): Allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation - 68-74°C Reaction Temperature: Reduces background amplification and improves primer specificity depending on the target - Lyophilization-ready: Component formulations enable generation of room temperature stable test kits through lyophilization
产品应用 - Simplify development of DNA loopmediated isothermal amplification (LAMP) reactions; - Optimization of DNA loopmediated isothermal amplification (LAMP) reactions.
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