Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE-Cyanine5, eBioscience™
货号：15-5981-82,15-5981-81
规格：100 µg,50 µg
价格：3585,0
产品类型：流式抗体
品牌：eBioscience
抗原：Sca-1
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：D7
荧光染料：PE-Cy5
类型: | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™ |
浓度： | 0.2 mg/mL | 用法： | 0.125 µg/test(Flow) |
Product Specific InformationDescription: The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationLy-6A/E molecules are implicated in the regulation of T cell activation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| Ly-6A/E (Sca-1) Antibody (15-5981-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.03 µg of Anti-Mouse Ly-6A/E (Sca-1) PE-Cyanine5.Total viable cells were used for analysis.Quadrants were set based on the autofluorescence sample. |
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参考文献： |
| 1.Stem cell reviewsInducible Nitric Oxide Synthase (iNOS) Is a Novel Negative Regulator of Hematopoietic Stem/Progenitor Cell Trafficking."15-5981 was used in Flow cytometry/Cell sorting to indicate that inducible nitric oxide synthetase is a negative regulator of hematopoietic cell migration."AuthorsAdamiak M,Abdelbaset-Ismail A,Moore JB,Zhao J,Abdel-Latif A,Wysoczynski M,Ratajczak MZ2.CellThe Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.3.Pain reportsIdentification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory abnormalities.4.LeukemiaEvidence that a lipolytic enzyme--hematopoietic-specific phospholipase C-β2--promotes mobilization of hematopoietic stem cells by decreasing their lipid raft-mediated bone marrow retention and increasing the promobilizing effects of granulocytes."15-5981 was used in Flow cytometry/Cell sorting to report the role of hematopoietic cell-specific phospholipase C-β2 (PLC-β2) in the pharmacological mobilization of hematopoietic stem/progenitor cells (HSPCs)."AuthorsAdamiak M,Poniewierska-Baran A,Borkowska S,Schneider G,Abdelbaset-Ismail A,Suszynska M,Abdel-Latif A,Kucia M,Ratajczak J,Ratajczak MZ5.Nature cell biologyRapid depot-specific activation of adipocyte precursor cells at the onset of obesity. |
QQ:1749072012