CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor 450, eBioscience™

货号：48-0495-82

规格：100 µg

价格：2296

产品类型：流式抗体

品牌：eBioscience

抗原：CD49f

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBioGoH3 (GoH3)

荧光染料：eFluor 450

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 450, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

Product Specific InformationDescription: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationThe ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD49f (Integrin alpha 6) Antibody (48-0495-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.06 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) eFluor® 450 (right). Total viable cells were used for analysis.

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参考文献：

1.Breast cancer research : BCRIntegrin-Rac signalling for mammary epithelial stem cell self-renewal.2.BloodDistinct signaling programs control human hematopoietic stem cell survival and proliferation."48-0495 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms by which distinct signalling programs control human hematopoietic stem cell survival and proliferation."AuthorsKnapp DJ,Hammond CA,Aghaeepour N,Miller PH,Pellacani D,Beer PA,Sachs K,Qiao W,Wang W,Humphries RK,Sauvageau G,Zandstra PW,Bendall SC,Nolan GP,Hansen C,Eaves CJ3.OncotargetLlgl1 prevents metaplastic survival driven by epidermal growth factor dependent migration.4.OncotargetYap1 promotes the survival and self-renewal of breast tumor initiating cells via inhibiting Smad3 signaling.5.Nature cell biologyOncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation."48049582 was used in flow cytometry to describe the role of mTOR signalling in recruiting pro-tumorigenic myeloid-derived suppressor cells."AuthorsWelte T,Kim IS,Tian L,Gao X,Wang H,Li J,Holdman XB,Herschkowitz JI,Pond A,Xie G,Kurley S,Nguyen T,Liao L,Dobrolecki LE,Pang L,Mo Q,Edwards DP,Huang S,Xin L,Xu J,Li Y,Lewis MT,Wang T,Westbrook TF,Rosen JM,Zhang XH

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