Nkx2.5 Polyclonal Antibody
货号：PA5-85215
规格：100 µL
价格：4375
产品类型：免疫组化一抗
品牌：iBiochips
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
类型: | 多抗 | 同型对照： | |
浓度： | 2.03 mg/mL | 用法： | 1:100-1:1000(ICC);1:100-1:1000(IF);Assay-dependent（IHC (P)）；1:500-1:3000(WB) |
免疫原性： | Recombinant protein encompassing a sequence within the center region of human Nkx2.5. | | |
产品详细信息Keep as concentrated solution.Positive Control: 293T, A549, mouse heart.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.靶标信息This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.
数据 |
| Nkx2.5 Antibody (PA5-85215) in WBWestern blot analysis of Nkx2.5 in various whole cell extracts using Nkx2.5 polyclonal antibody (Product # PA5-85215) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.Nkx2.5 Antibody (PA5-85215) in ICCImmunocytochemistry analysis of Nkx2.5 in 4% paraformaldehyde-fixed human embryonic stem cells (D3) using Nkx2.5 polyclonal antibody (Product # PA5-85215) at a dilution of 1:200. |
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