LavaLAMP™ DNA Component Kit with Dye/LavaLAMP™DNA组件试剂盒含染料
货号：30077-1
规格：500 Reactions
价格：3802
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The LavaLAMP™ DNA Component Kit is intended to simplify development and optimization of DNA loopmediated isothermal amplification (LAMP) reactions. This kit is for research purposes only, and available under the limited-use license described at the end of this document. LAMP commonly employs a set of six primers (F3, B3, Loop-F, Loop-B, FIP, and BIP), which must be supplied by the user. Previously-established primer designs may be used. Alternatively, Lucigen recommends use of the free Eiken web utility or the commercially available LAMP Design Software from Premier BioSoft to design new primer sets (see Appendix A). Not all primer sets identified by these programs are guaranteed to work with LavaLAMP™ DNA Component Kit or any other LAMP system. We strongly encourage designing multiple primer sets to identify the best performing set. We also highly recommend inclusion of loop primers (Loop-F and Loop-B; Nagamine, 2002) to improve assay performance. LavaLAMP™ DNA amplification products may be detected by agarose gel electrophoresis or by realtime/end-point monitoring with fluorescent double-stranded DNA-binding dyes, such as the Lucigen Green Fluorescent Dye (Cat. No. 30078-1). Turbidity may also be monitored to assess amplification (Mori, 2001), but this method is less sensitive. LavaLAMP™DNA组分试剂盒旨在简化DNA环介导的等温扩增（LAMP）反应的开发和优化。该试剂盒仅用于研究目的,可根据本手册末尾所述的有限使用许可使用。 LAMP通常使用一组六个引物（F3，B3，Loop-F，Loop-B，FIP和BIP),这些引物必须由用户提供。可以使用先前建立的引物设计。另外，Lucigen建议使用免费的Eiken Web实用程序或Premier BioSoft的市售LAMP Design软件来设计新的引物组（请参阅附录A)。不能保证这些程序确定的所有引物都可与LavaLAMP™DNA组分试剂盒或任何其他LAMP系统一起使用。我们强烈建议设计多个引物组，以鉴定表现最佳的组。我们还强烈建议加入环引物（Loop-F和Loop-B； Nagamine，2002）以提高测定性能。 LavaLAMP™DNA扩增产物可通过琼脂糖凝胶电泳或荧光双链DNA结合染料（如Lucigen Green荧光染料（货号30078-1））进行实时/终点监测来检测。 也可以监测浊度以评估扩增（Mori，2001）,但是这种方法敏感性较低。 |
| 优点： |
| ▪ Loop-mediated Isothermal Amplification (LAMP): Allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation▪ 68-74°C Reaction Temperature: Reduces background amplification and improves primer specificity depending on the target▪ Lyophilization-ready: Component formulations enable generation of room temperature stable test kits through lyophilization▪ Custom/Bulk Available: Custom volumes and dispensing available to match your specific requirements ▪ 环形-介导的等温扩增（LAMP）：允许研究人员使用低复杂度的仪器在高科技实验室之外进行测定。 ▪ 68-74°C反应温度：减少背景扩增并根据靶标提高引物特异性▪ 冻干准备就绪：成分配方可通过冻干生成室温稳定的测试试剂盒 |
| 数据： |
| Figure 1. Positive LAMP Reaction Products from a Positive Control Target. Lane 1: 100 bp Marker, Lane 2: No Target Control reaction. Lanes 3 and 4: A distinct banding pattern is seen among the smear, which is indicative of a positive LAMP reaction. 图 1.来自阳性对照靶的阳性LAMP反应产物。 泳道1：100bp标记，泳道2：无靶对照反应。 泳道3和4：在涂片中看到明显的条带图案，这表明LAMP反应为阳性。 Figure 2.Typical Background Amplification in a LAMP Reaction. Lane 1: 100 bp ladder, Lanes 2 and 3: Non-specific or background amplification appears as a smear of DNA fragments with no visible or distinct bands. A prominent primer dimer band is also characteristic of non-specificamplification. Lane 4: Absence of non-specific amplification (no products). 图 2. LAMP反应中的典型背景扩增。 泳道1：100 bp阶梯，泳道2和3：非特异性或背景扩增表现为DNA片段涂片，无可见或明显条带。 突出的引物二聚体带也是非特异性扩增的特征。 泳道4：不存在非特异性扩增（无产物）。Fluorescent Signals from Different LAMP Reactions 来自不同LAMP反应的荧光信号Figure 3. Early Fluorescent Signals from Positive LAMP Reactions and Late Background Signals from NTC Controls after Extended Incubation. LAMP reactions were run in a real-time thermal cycler. The fluorescent signal from each reaction was captured over a 60-minute reaction time. The red and blue lines represent the fluorescent signals from Positive Control reactions with varying amounts of target. The black lines represent the non-specific background amplification signals that arise later in No Target Control reactions.图3. 长时间孵育后，来自阳性LAMP反应的早期荧光信号和来自NTC对照的晚期背景信号。LAMP反应在实时热循环仪中进行。在60分钟的反应时间内捕获了每个反应的荧光信号。红线和蓝线表示来自阳性对照反应的荧光信号，靶标量不同。黑线表示非特异性背景扩增信号，该信号稍后在“无靶标对照”反应中出现。 |
| 组成成分： |
| ▪ 10X LavaLAMP™ DNA Buffer ：储存在-20°C▪LavaLAMP™ DNA Enzyme ：20μL/次；储存在2-8°C▪Magnesium Sulfate, 100mM ：储存在-20°C▪DNA Positive Control LAMP Primer Mix ：- 20°C▪DNA Positive Control ：储存在-20°C |
| 相关产品： |
| ▪ LavaLAMP™ DNA Component Kit （货号#30076-1）▪ Green Fluorescent Dye（货号#30078） |
| 参考文献： |
| Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000 28(12):E63. Nagamine K, Hase T, Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002 16(3):223-9. |
技术参数 产品优点 - Loop-mediated Isothermal Amplification (LAMP): Allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation - 68-74°C Reaction Temperature: Reduces background amplification and improves primer specificity depending on the target - Lyophilization-ready: Component formulations enable generation of room temperature stable test kits through lyophilization- 环形-介导的等温扩增（LAMP）：允许研究人员使用低复杂度的仪器在高科技实验室之外进行测定。- 68-74°C反应温度：减少背景扩增并根据靶标提高引物特异性- 冻干准备就绪：成分配方可通过冻干生成室温稳定的测试试剂盒
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