SUMO Express Protease 蛋白酶
货号：30801-2
规格： 200 U
价格：3532
产品类型：蛋白提取/纯化
品牌：Lucigen
| SUMO Express Protease is a highly active and robust engineered protease derived from the yeast
ULP1 gene. It is used to remove the SUMO tag from recombinant SUMO-tagged fusion proteins
after purification. It has been specifically engineered to recognize and cleave the stabilized form of
the SUMO tag used in Lucigen’s Expresso™ SUMO Cloning and Expression kits. This stabilized
SUMO tag contains amino acid substitutions that render it resistant to desumoylation enzymes
present in eukaryotic cells, enabling expression of SUMO fusion proteins in insect or mammaliancells. The SUMO Express Protease contains compensatory mutations that enable it to recognize
and cleave the stabilized SUMO tag. Other commercially available SUMO proteases lacking these
compensatory mutations cannot cleave the SUMO tag used in the Expresso SUMO kits. SUMO
Express Protease exhibits activity against the native (nonstabilized) SUMO tag, but at a reduced
rate compared to native SUMO protease. SUMO Express protease is 6xHis tagged, allowing
convenient removal of the protease after cleavage by metal affinity chromatography. |
| 相关产品： |
| ▪Expresso® Rhamnose SUMO Cloning and Expression System（货号#49013）▪Expresso® T7 SUMO Cloning and Expression System（货号#49003） |
技术参数 产品优点 - highly active
产品应用 - SUMO Express Protease is used to remove the SUMO tag from recombinant SUMO-tagged fusion proteins after purification.
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