CloneSmart LCKan Blunt Cloning Kit（停产）
货号：40821-2,40821-4
规格：20 rxns ,40 rxns
价格：4123,7443
产品类型：二代测序-建库
品牌：Lucigen
| Lucigen’s unique cloning kits with pre-processed vectors and highly competent cells eliminate hours of tedious preparation and enable efficient cloning. Ligation can be completed in as little as 30 minutes with no clean-up needed; only a brief heat denaturation is necessary before transformation. Downstream manipulation (e.g, mutagenesis, subsequent insert addition) is facilitated by the minimal vector size (1.7-2.0 kb). |
| 优点： |
| ▪ Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation；▪ Transcription terminators flanking cloning site stabilize otherwise toxic inserts；▪ Choice of copy number and antibiotic resistance. |
| 数据： |
| Figure 1.High copy (HC) and low copy (LC) versions of the pSMART transcription-free cloning vectors. |
| 相关产品： |
| ▪CloneSmart HCKan Blunt Cloning Kit（货号#40704）▪ CloneSmart LCAmp Blunt Cloning Kit（货号#40300）▪CloneSmart HCAmp Blunt Cloning Kit（货号#40041） |
技术参数 产品优点 - Convenient, pre-processed vectors eliminate the need for digestion, gel purification, and dephosphorylation.
- Transcription terminators flanking cloning site stabilize otherwise toxic inserts.
- Choice of copy number and antibiotic resistance.
组成成分 - 4X CloneSmart Vector Premix Includes Buffer, ATP, and one type of ligation-ready Vector
- CloneSmart DNA Ligase (2 U/µL)
- Positive Control Insert DNA (500 ng/µL lambda HincII)
- CloneSmart Sequencing Primers (200 reactions each)
SL1 Primer (3.2 pmol/µL)
SR2 Primer (3.2 pmol/µL)
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