T4 Polynucleotide Kinase, Cloned T4多核苷酸激酶，克隆
货号：P0503K
规格：3000 U
价格：2890
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| T4 Polynucleotide Kinase, Cloned (T4 PNK) catalyzes the transfer of the γ-phosphate of ATP to the 5′ terminus of single- and double-stranded DNA or RNA molecules that have a 5′ hydroxyl. The enzyme also removes the 3′ phosphate from 3′-phosphoryl polynucleotides, deoxyribonucleoside 3′-monophosphates, and deoxyribonucleoside 3′,5′-diphosphates to form a 3′ hydroxyl. T4 PNK has many uses, including labeling the 5′ ends of DNA or RNA with 32P for use in sequencing, as hybridization probes, or in transcript mapping with S1 nuclease or Mung Bean Nuclease. The enzyme can also be used to phosphorylate oligodeoxynucleotide linkers or other DNA molecules prior to ligation and to end-label oligodeoxynucleotide primers for use in sequencing reactions. T4 PNK may also be used in an exchange reaction to radiolabel the 5′ ends of DNA or RNA molecules that have unlabeled 5′ phosphates. It also may be used to dephosphorylate the 3′-ends of RNA in the absence of ATP. T4多聚核苷酸激酶（T4 PNK）催化ATP的γ-磷酸转移到单链和双链DNA或具有5，-羟基的RNA分子的5，末端。该酶还可从3，-磷酰基多核苷酸、脱氧核糖核苷3-单磷酸酯和脱氧核糖核苷3，，5-二磷酸中除去3，磷酸，形成3-OH基因 |
| 优点： |
| ▪ Robust: Highly active enzyme that readily phosphorylates 5' hydroxl ends of nucleic acids▪ Flexible: Use newly phosphorylated nucleic acids in a variety of applications such as cloning, next gen sequencing library prep, and preparation of labeled nucleic acids |
| 组成成分： |
| ▪ T4 PNK (10 U/µL)：储存在-20°C▪ 10X T4 PNK Buffer：储存在-20°C |
技术参数 产品优点 - Robust: Highly active enzyme that readily phosphorylates 5' hydroxl ends of nucleic acids
- Flexible: Use newly phosphorylated nucleic acids in a variety of applications such as cloning, next gen sequencing library prep, and preparation of labeled nucleic acids
产品应用 - Labeling the 5′ ends of DNA or RNA with 32P for use in sequencing；
- Hybridization probes, or in transcript mapping with S1 nuclease or Mung Bean Nuclease；
Phosphorylate oligodeoxynucleotide linkers or other DNA molecules prior to ligation and to end-label oligodeoxynucleotide primers for use in sequencing reactions.
- T4 PNK may also be used in an exchange reaction to radiolabel the 5′ ends of DNA or RNA molecules that have unlabeled 5′ phosphates.
- To dephosphorylate the 3′-ends of RNA in the absence of ATP.
- 标记DNA和RNA的5'末端用于DNA测序、印迹杂交或转录本作图
- 连接反应前对寡核苷酸接头和其他DNA或RNA分子进行磷酸化，或用于Ampligase热稳定DNA连接酶介导的连接扩增反应
- 制备DNA或RNA分子量的标准品用于凝胶电泳和色谱分析
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