Endura™ Chemically Competent Cells/Endura™化学感受态细胞
货号：60240-1 ,60240-2,60241-1
规格：12 rxns (DUO) ,24 rxns (DUO) ,12 rxns (SOLOs)
价格：2453,4317,2694
产品类型：感受态细胞
品牌：Lucigen
| Lucigen’s Endura Competent Cells are noted for their ability to clone and maintain plasmid DNA
sequences that are otherwise unstable, such as those with inverted repeats or sequences that are
otherwise prone to undesired recombination events.
Such unstable inserts are commonly found in retroviral DNA, and can prove exceptionally difficult to
isolate and propagate in traditional cloning strains. Lucigen’s Endura Competent Cells are available in
high efficiency chemically competent or electrocompetent versions to make difficult cloning work more
reliable and efficient. Lucigen的Endura感受态细胞具有克隆和维持原本不稳定的质粒DNA序列的能力，例如具有反向重复序列或原本容易发生非理想重组事件的序列。 这种不稳定的插入片段通常在逆转录病毒DNA中发现，并证明在传统克隆菌株中很难分离和繁殖。 Lucigen的Endura感受态细胞具有高效的化学感受态或电感受态形式，可以使困难的克隆工作更加可靠和高效。 |
| 优点： |
| ▪ Stabilize direct repeats and create lentiviral constructs；▪Generatelentiviral guide RNA libraries；▪Recommended inCRISPR GeCKO library protocols；▪稳定直接重复序列并创建慢病毒构建体稳定直接重复序列并创建慢病毒构建体▪生成慢病毒指南RNA库▪CRISPR GeCKO文库操作推荐 |
| 数据： |
| Figure 1.Cost per reaction of chemically competent cells of clone-stabilizing strains from other suppliers.图1.其他供应商克隆稳定菌株的化学感受态细胞的每次反应成本。Figure 2.Transformation efficiencies of electrocompetent clone-stabilizing strains.图2.电感受态克隆稳定菌株的转化效率。 |
| 相关产品： |
| ▪Endura™ ElectroCompetent Cells（货号#60242）▪ Recovery Medium（货号#80026-1） |
技术参数 产品优点 - Stabilize direct repeats and create lentiviral constructs
稳定直接重复序列并创建慢病毒构建体稳定直接重复序列并创建慢病毒构建体
- Generate lentiviral guide RNA libraries
生成慢病毒指南RNA库
- Recommended in CRISPR GeCKO library protocols
CRISPR GeCKO文库操作推荐
- Choose electrocompetent or chemically competent cells
选择电感受态或化学感受态细胞
- Highest efficiency commercially available cells for lentiviral cloning: over 1 × 10^7 cfu/µg (chem) or 1 × 10^10 cfu/µg (electro)
商业上可获得的效率最高的慢病毒克隆细胞：超过1×10^7 cfu / µg（化学）或1×10^10 cfu / µg（电）
产品应用 - Clone repetitive sequences and lentiviral libraries
克隆重复序列和慢病毒文库
组成成分 Endura™ Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.
Endura™感受态细胞包括对照DNA和恢复培养基，并按照指示包装为SOLO（每管1个转化）或DUO（每管2个转化）。 Endura Electrocompetent细胞仅以DUO形式提供。 还可以单独使用恢复培养基。 除非另有说明，否则指定的转化效率适用于pUC DNA。
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