msgbst cDNA sysKit for qPCR/msgbst qPCR DNA合成试剂盒（停产））
货号：MB060124
规格：24 Rxns
价格：7320
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The MessageBOOSTER™ cDNA Synthesis Kit for qPCR reaction amplifies the poly(A) RNA (mRNA) in a total RNA sample isolated from 1-50 eukaryotic cells to enable more sensitive and more reproducible qPCR of even low-abundance transcripts. Note: MessageBOOSTER is not suited for bacterial/prokaryotic total RNA samples. 用于qPCR反应的MessageBOOSTER™cDNA合成试剂盒可扩增从1-50个真核细胞中分离出的总RNA样品中的poly（A）RNA（mRNA），从而即使是低丰度的转录本，也可以实现更灵敏和可重复的qPCR。 注意：MessageBOOSTER不适合细菌/原核总RNA样品。 |
| 优点： |
| ▪ Perform RNA amplification on purified total RNA followed by cDNA synthesis and detection ▪ Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.▪ Get more data out of precious samples – use less RNA for more RT-qPCR reactions▪ Readily and reproducibly detect even low-abundance transcripts in RNA from a single cell (CT values <35 cycles) ▪ Collect small RNA samples less often. ▪ Fast protocol amplifies and synthesizes cDNA in only 1 day. ▪ 对纯化的总RNA进行RNA扩增，然后进行cDNA合成和检测；▪ 使用这种高保真度的线性RNA扩增过程进行扩增，可保留样品的相对转录本丰度；▪ 从珍贵样品中获取更多数据–使用更少的RNA进行更多的RT-qPCR反应；▪ 从单个细胞中即可简便且可重现地检测RNA中的低丰度转录物（CT值<35个循环）；▪ 较少收集小RNA样品；▪ 快速操作，仅需1天即可扩增并合成cDNA。 |
| 数据： |
| Figure 1. Protocol overview of the MessageBOOSTER™ cDNA Synthesis Kits.Each MessageBOOSTER kit reaction amplifies the poly(A) RNA (mRNA) directly from either (A) purified total RNA or (B) crude cell lysates. The amplified RNA is then reverse-transcribed to cDNA that can be diluted up to 1,000-fold for qPCR. 图1. MessageBOOSTER™cDNA合成试剂盒的方案概述。每个MessageBOOSTER试剂盒反应都直接从（A）纯化的总RNA或（B）粗细胞裂解物中扩增poly（A）RNA（mRNA）。 然后将扩增的RNA反转录为cDNA，可以将其稀释至1000倍以进行qPCR。 Figure2.qPCR results from cDNA produced from a single NRK cell.cDNA was made from a single NRK cell using the MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit and Qpcr of the PBGD target was performed using undiluted(red),1:10 diluted (green),1:100 diluted (blue),and 1:1,000 diluted(purple) aliquots.The low-abundance PBGD transcript was readily detected.图2.qPCR结果来自单个NRK细胞产生的cDNA。使用MessageBOOSTER™cDNA从Cell Lysates Kit合成单个NRK细胞，进行PBGD靶标的Qpcr：使用未稀释（红色），1：10稀释（绿色），1：100稀释（蓝色）1 ：1,000稀释的（紫色）等分试样。低丰度PBGD转录本易于检测。 |
| 组成成分： |
| ▪T7-Oligo(dT) Primer A：-20°C▪ RiboGuard RNase Inhibitor (40 U/µL) ：-20°C▪ RT PreMix：-20°C▪ DNA Polymerase PreMix：-20°C▪ DNA Polymerase：-20°C▪ Finishing Solution：-20°C▪ Random Primers：-20°C▪ RNase H：-20°C▪ MMLV-RT：-20°C▪ IVT PreMix A：-20°C▪ T7 RNA Polymerase：-20°C▪ 10X Transcription Reaction Buffer：-20°C▪ RNase-Free DNase I (1 U/µL)：-20°C▪ Forward Control PCR Primer (12.5 µM)：-20°C▪ Reverse Control PCR Primer (12.5 µM)：-20°C▪ DTT (100 mM)：-20°C▪ Nuclease-Free Water, Sterile：-20°C▪ Poly(I)：-20°C▪ NRK Total RNA Control (50 ng/µL)：-80°C |
| 参考文献： |
| 1.Van Gelder, R. N. et al., (1990) Proc. Natl. Acad. Sci. USA 87 (5), 1663. |
技术参数 产品优点 - Perform RNA amplification on purified total RNA followed by cDNA synthesis and detection
- Amplify with this high-fidelity, linear RNA amplification process that preserves the relative transcript abundance of the sample.
- Get more data out of precious samples – use less RNA for more RT-qPCR reactions
- Readily and reproducibly detect even low-abundance transcripts in RNA from a single cell (CT values <35 cycles)
- Collect small RNA samples less often.
- Fast protocol amplifies and synthesizes cDNA in only 1 day.
-对纯化的总RNA进行RNA扩增，然后进行cDNA合成和检测；
-使用这种高保真度的线性RNA扩增过程进行扩增，可保留样品的相对转录本丰度；
-从珍贵样品中获取更多数据–使用更少的RNA进行更多的RT-qPCR反应；
-从单个细胞中即可简便且可重现地检测RNA中的低丰度转录物（CT值<35个循环）；
-较少收集小RNA样品；
-快速操作，仅需1天即可扩增并合成cDNA。
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18519.htm
QQ:1749072012