Pierce TiO2 Phosphopeptide Enrichment Spin Tips TiO2磷酸肽富集旋转吸头
货号：88303
规格：96 tips
价格：15552
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Pierce TiO2 Phosphopeptide Enrichment Spin Tips are 200µL pipette tips containing TiO2 resin. This product is also sold as part of the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit. |
| 数据： |
| TiO2 and Fe-NTA IMAC Resins enrich a complementary set of phosphopeptidesThe Venn diagram details the number of unique phosphopeptides identified from 250µg of peptides prepared from nocodazole-treated U2OS cells. Phosphopeptides were enriched with the Pierce TiO2 Phosphopeptide Enrichment and Clean-up Kit and the Pierce Fe-NTA IMAC Phosphopeptide Enrichment Kit. Eluted peptides were desalted and concentrated with Graphite Spin Columns before LC-MS/MS analysis on a Thermo Scientific Orbitrap XL Mass Spectrometer. Data was analyzed with Thermo Scientific Proteome Discoverer 2.1 and Proteome Software Scaffold 3.0.TiO2 selectively enriches more phosphopeptides than Fe-NTA IMACThe numbers refer to the total and unique number of phosphopeptides enriched by each method. The Y-axis is the percentage of phosphopeptides in the number of total and unique peptides, or the percentage of acidic residues in enriched unique phosphopeptides. Selective enrichment of singly and multiply phosphorylated phosphopeptidesAverage phosphopeptide enrichment results from duplicate experiments showing the number of phosphopeptides containing one to six (center columns) phosphates per peptide enriched using Thermo Scientific Pierce TiO2 and FE-NTA IMAC resins. Peptide spectrum summary results were exported from Proteome Software Scaffold 3.0. |
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