Pierce Protein A IgG Plus Orientation Kit, 2 mL 蛋白 A IgG 加方向试剂盒，2 mL
货号：44893
规格：2 columns
价格：9921
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| The Thermo Scientific Pierce A IgG Plus Orientation Kit uses a crosslinker to covalently attach antibody that is bound to Protein A agarose resin, thereby creating a reusable affinity column for purification of antigens.This "Plus" kit provides for more efficient and stable antibody immobilization than the traditional orientation method because it uses the NHS-ester crosslinker DSS (disuccinimidyl suberate) rather than the imidoester crosslinker DMP (dimethyl pimelimidate). Several milligrams of IgG can be bound and crosslinked to each 2 ml column, after which the prepared affinity column can be used multiple times for antigen purification using typical binding and elution conditions. |
| 特点： |
| - Contaminant-free antigen purification—provides for antigen purification without contamination (co-elution) of antibody- Covalent, leak-resistant antibody immobilization—high-capacity binding of IgG to Protein A agarose, followed by efficient crosslinking with DSS crosslinker- High-quality agarose resin—crosslinked 6% beaded agarose with recombinant Protein A- Gentle binding and crosslinking conditions—coupling antibody in standard PBS- Oriented immobilization—binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance- Complete kit—includes all the reagents needed to immobilize the antibody- Better than DMP—less antibody leaching than traditional orientation method with DMP crosslinker |
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