Pierce Protein A Coated Plates, Clear, 8-Well Strip 蛋白A包被8孔板，透明
货号：15132
规格：5 plates
价格：4072
产品类型：ELISA检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| Thermo Scientific Pierce Protein A Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.These plates are uniformly and stably coated with Protein A, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA. |
| 特点： |
| - Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption- Orient antibodies for maximum antigen-binding capacity- Immobilize antibodies for plate assays without prior purification- Ensure minimal variation (<5% well-to-well) from consistent coating- Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer- Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG) |
| 数据： |
Greater signal compared to directly coated antibodyComparison of signal obtained by antibody bound to Protein A and Protein G coated plates, and antibody passively bound to polystyrene plates. Goat Anti-Biotin was serially diluted across Protein A, Protein G and polystyrene plates. The Goat Anti-Biotin was detected by the addition of biotin, followed by streptavidin-HRP and finally TMB substrate. |
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