Ulysis Alexa Fluor 647 Nucleic Acid Labeling Kit/Alexa Fluor 647核酸标记试剂盒
货号：U21660
规格：1 kit
价格：8053
产品类型：核酸标记
品牌：Thermo Fisher
| ULYSIS Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast—just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.Reliable Labeling With the Universal Linkage SystemWe developed this series of ULYSIS kits to enable rapid and simple coupling of our Alexa Fluor dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS), is based on the use of a platinum dye complex (owned by KREATECH Diagnostics) that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.Labeling is Fast and EasyThe labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS complex can be accomplished through the use of a simple spin-column procedure. DNA longer than ~1,000 base pairs requires a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization. |
| 数据： |
| |
| 参考文献： |
| 1.A custom microarray platform for analysis of microRNA gene expression.2.A systematic search for new mammalian noncoding RNAs indicates little conserved intergenic transcription.3.Expression profile analysis of microRNA (miRNA) in mouse central nervous system using a new miRNA detection system that examines hybridization signals at every step of washing. |
技术参数 产品优点 • Dye (Ex/Em): Alexa Fluor® 647 (650/670 nm)
• Labeling reaction is complete in as little as 15 minutes
• Available in several Alexa Fluor® dye colors
产品应用 • Dot, northern, and Southern blots
• RNA and DNA in situ hybridization
• Multicolor fluorescence in situ hybridization (mFISH)
• Comparative genome hybridization (CGH)
• Microarray analysis
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18875.htm
QQ:1749072012