Pierce Maleimide Activated Plates, White, 96-Well 马来酰亚胺包被96孔板，白色
货号：15152
规格：5 plates
价格：3032
产品类型：ELISA检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| These Thermo Scientific Pierce Maleimide Activated White, 96-Well Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. Our coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production.Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane-HCl (Traut's Reagent). |
| 特点： |
| - White, for chemiluminescent assays- Pre-blocked to reduce nonspecific binding- Easy (spontaneous) immobilization of peptides derivatized with a terminal cysteine and proteins with free sulfhydryl- Binding Capacity: 100 to 150 pmol of a sulfhydryl-containing peptide (307 Da) per well- Activation Level: 100 µL |
| 数据： |
Reaction scheme for coupling peptides and other sulfhydryl-containing molecules |
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