Pierce Iminobiotin Agarose 异生物素琼脂糖
货号：20221
规格：5 mL
价格：2854
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Thermo Scientific Pierce Iminobiotin Agarose provides for unusual affinity purification experiments involving clean-up or removal of streptavidin or avidin protein components.Pierce Iminobiotin Agarose is beaded agarose support onto which iminobiotin moieties have covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin is needed to capture avidin or streptavidin components from a sample. This iminobiotin resin addresses this special situation.Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Because no denaturing agents such as 8M guanidine hydrochloride or 4M urea are required for elution, the avidin conjugate has a better chance of maintaining its activity after purification. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components. |
| 特点： |
| - Enable purification or removal of avidin or streptavidin samples- Crosslinked beaded agarose onto which iminobiotin is covalently immobilized- Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin) |
| 数据： |
Chemical structure of Iminobiotin AgaroseEach porous agarose bead is 45 to 165µm diameter and contains trillions of iminobiotin groups. |
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