Pierce Streptavidin Coated High Sensitivity Plates, Black, 8-Well Strip 链霉亲和素包被高灵敏度8孔黑色板
货号：15525
规格：5 plates
价格：5121
产品类型：ELISA检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| Thermo Scientific Pierce Streptavidin Coated High Sensitivity Plates,Black, 8-Well Strip are biotin-binding microplates that are coated to maximize capture efficiency of biotinylated probes and control background in assays for low levels of target analyte.Thermo Scientific Pierce Coated Plates are coated using proprietary surface-activation technology to achieve maximum efficient coating of active streptavidin biotin-binding protein. The high sensitivity plates enable detection of low concentrations (ng/mL) of biotinylated antibodies and other proteins. Whether to detect signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.Streptavidin is the most popular and widely available biotin-binding protein for research methods. Streptavidin has an isoelectric point of 5 to 6, resulting in low non-specific interactions. |
| 特点： |
| - Sensitive—detect down to 5 ng/mL of biotinylated IgG- Specific—low background with high signal-to-noise ratios- Versatile—use either fluorescence, chemiluminescence or colorimetric detection- Robust—broad dynamic range- Ready to use—plates are supplied pre-blocked to save time |
| 数据： |
Detect low concentrations of biotinlyated target molecules using High Sensitivity Coated PlatesBiotinylated rabbit IgG was incubated for 30 minutes in streptavidin-coated high sensitivity plates (HS) or streptavidin-coated standard binding capacity (SBC) microplates. Plates were washed three times with Tris-buffered saline and incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (100ng/mL) for 30 minutes. Plates were washed and incubated with Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate (Part No. 15169). The fluorescent signal was measured at 430nm. Comparison of Streptavidin Coated Plate typesDetection Ranges were determined using Thermo Scientific QuantaBlu Fluorogenic Peroxidase Substrate. |
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