Pierce High Sensitivity NeutrAvidin-HRP 高灵敏度中性亲和素

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Pierce High Sensitivity NeutrAvidin-HRP 高灵敏度中性亲和素

货号:31030,31032

规格:0.5 mL,5 mL

价格:3408,16969

产品类型:免疫印迹/组化

品牌:Thermo Fisher

Thermo Scientific Pierce 高灵敏度 NeutrAvidin HRP 偶联物是经过特殊制备的过氧化物酶偶联形式的亲和素生物素结合蛋白,用于提供信号扩增(如多聚-HRP)和优异的储存稳定性。这种经过特殊制造的 HRP 偶联亲和素蛋白可满足当今科学家对 ELISA 和 Western 印迹应用中更高检测灵敏度的要求。该偶联物适用于化学发光、化学荧光或比色底物。每种高灵敏度 HRP 偶联物均采用易于使用的稳定溶液包装,以便能够在 4°C 条件下至少储存一年。我们的高灵敏度 HRP 偶联物在靶标检测、信号强度和信噪比方面优于竞争产品。例如,实验表明,与传统 HRP 偶联物相比,高灵敏度链霉亲和素-HRP 可多稀释八至 10 倍,但仍可产生相似的结果。
特点:
-NeutrAvidin 蛋白—一种特殊去糖基化形式的亲和素,在许多应用中提供生高度特异性、低背景的物素化抗体结合。-高灵敏度—可在无背景条件下检测出低水平的靶标;获得高信噪比-经济实惠—在 Western 印迹和 ELISA 应用中使用较少偶联物,但仍可获得优异的结果-灵活—兼容典型的化学发光、荧光和比色过氧化物酶底物-便利—即用型稳定液体形式,这意味着无需等待解冻,也无需等分
数据:

Excellent IHC staining of GAPDH and cytokeratin 18 in human colon carcinoma with High Sensitivity HRP ConjugatesFormalin-fixed paraffin-embedded human colon carcinoma tissues were stained using Metal Enhanced DAB Substrate (Thermo Scientific Pierce Immunohistochemistry Peroxidase Detection Kit, Part No.36000). The tissues were incubated with either biotinylated anti-GAPDH or rabbit anti-cytokeratin18 followed by biotinylated anti-rabbit IgG (left panels) or blocking buffer only (right panels). The sections were subsequently incubated with Thermo Scientific High Sensitivity NeutrAvidin-HRP (top panels) or Streptavidin-HRP (bottom panels). Tissues were counterstained using the Harris-modified hematoxylin solution (blue staining in all panels). GAPDH and Cytokeratin18, stained using the NeutrAvidin HRP and streptavidin HRP, appear brown (left panels) while the Negative Control panels shows no brown staining in the tissue.

技术参数
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