Pierce Chicken IgY Purification Kit / 鸡IgY纯化试剂盒
货号：44918,44922
规格：1 L,5 L
价格：5381,19075
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| The Thermo Scientific Pierce Chicken IgY Purification Kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.Typical IgG affinity resins (Protein A or Protein G) do not bind chicken IgY and therefore are not effective for IgY purification. The Pierce Chicken IgY Purification Kit uses a delipidation-and-precipitation procedure that is optimized to recover approximately 100 mg of 90% pure IgY from fresh egg yolks of appropriately immunized chickens. The resulting polyclonal IgY is sufficiently pure for direct use in many immunodetection procedures, or it can be easily affinity-purified against specific antigen because it is dissolved in PBS and thoroughly clarified.Affinity chromatography with Protein A or Protein G cannot be used to purify IgY from chicken serum or egg yolk because this species of immunoglobulins are not recognized by the Protein A, G or L binding sites. However, the Pierce Chicken IgY Purification Kit provides a simple and reliable method for recovering large amounts of chicken IgY from egg yolk with our unique delipidation reagent that separates the proteins from lipid. (The egg yolk can be stored in the delipidation reagent a year or longer before processing.) After lipid separation, simply mix the sample with the IgY Precipitation Reagent which causes the IgY to separate from the lipid fraction during centrifugation. The precipitated chicken IgY is then easily recovered by dissolution in phosphate-buffered saline (PBS). |
| 特点： |
| - More for your money—purifies twice the amount of IgY than the leading competitor's kit with a lower cost-per-mg of IgY purified- High recovery and purity—obtain 80 to 120 mg IgY per egg yolk at 85% to 95% purity by SDS-PAGE analysis- Ease-of-use—simple precipitation method purifies chicken IgY without affinity columns- Trouble-free method—fewer steps than other IgY purification kits- Flexibility—eggs can be stored in buffer and purified at a later date- Convenience—use eggs directly out of refrigerator; no need to wait for them to warm up |
| 数据： |
Yolks and whites are separated (1). Delipidation reagent is added (2), followed by centrifugation (3) and separation from lipid solids. Then, IgY precipitation reagent is added (4), followed by centrifugation to pellet the IgY (5). After the liquid is decanted, the purified IgY is dissolved in phosphate-buffered saline (6). |
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