Pierce Albumin Depletion Kit / 白蛋白去除试剂盒
货号：85160
规格：24 reactions
价格：3495
产品类型：蛋白凝胶电泳
品牌：Thermo Fisher
| Pierce Albumin Depletion Resin is supplied as a 50% slurry. Dispense 200 µL of slurry into the supplied microcentrifuge spin columns to obtain 100 µL of settled beads for the standard protocol. Each aliquot of resin can be used to process 10 to 50 µL of serum sample in a single reaction. Processed samples are ready for immediate downstream analysis by electrophoresis or MS applications. The ease and versatility offered by the slurry format make this kit ideal for single- or multi-sample processing with the microcentrifuge spin columns supplied in the kit. 人血清白蛋白（HSA）通常占血清样品中总蛋白的60％以上，浓度约为40 mg / mL。高浓度的白蛋白阻碍了研究，使许多生物学上感兴趣的蛋白检测难以进行。传统上，研究人员使用涉及多个纯化步骤的层析法生产不含白蛋白的样品。除了涉及冗长而乏味的纯化过程之外，这些纯化步骤还倾向于降低蛋白产量。白蛋白去除试剂盒是为利用了Cibacron *蓝染料结合白蛋白的特性而开发的。 该试剂盒经过优化，可结合血清样品中的人、猪和绵羊白蛋白。通过对方案的优化，可以用这种方法去除小牛、牛、山羊和大鼠的白蛋白。该试剂盒不能有效去除小鼠白蛋白。 Human serum albumin (HSA) often accounts for greater than 60% of the total protein present in serum samples and can have a concentration of approximately 40 mg/mL. The high concentration of albumin hinders research, obscuring the detection of many proteins of biological interest. Traditionally, researchers have produced albumin-free samples using chromatography methods involving multiple purification steps. In addition to involving lengthy and tedious procedures, these purification steps also tend to give low protein yields. The Pierce Albumin Depletion Kit was developed to take advantage of the Cibacron* Blue Dye albumin binding properties.The kit is optimized to bind human, porcine and sheep albumin from serum samples. With a modification to the protocol, albumin from bovine, calf, goat and rat can be removed with this method. The kit is not effective for removal of mouse albumin. |
| 特点： |
| - Complete kit—includes optimized buffer and microcentrifuge spin columns to remove albumin quickly and conveniently from 10 to 50 µL samples- Easy-to-dispense slurry—improves performance, eases processing of multiple samples, and can be adapted to larger or smaller columns or 96-well filter plates- Workflow compatible—removing excess albumin facilitates MS or electrophoresis gel analysis of low-abundance serum proteins |
| 数据： |
Effective albumin removal improves 2D gel analysis of serumHuman serum was diluted five-fold in TBS (i.e., 10μL of serum added to 40μL of TBS), and 5μL of the diluted serum was separated by 2D-SDS PAGE. B. Human serum was diluted two-fold in Binding/Wash Buffer (i.e., 50μL of serum added to 50μL of buffer) processed using the Pierce Albumin Depletion Kit according to the instructions. Albumin-depleted samples and washes were combined (i.e., 100μL sample with 150μL wash buffer) and 5μL was separated by 2D-SDS PAGE. For 2D-SDS PAGE analysis, samples were diluted with 2D SDS-PAGE loading buffer, focused using pH 4-7 isoelectric focusing (IEF) strips and separated using 8-16% Tris-glycine gels. Gels were stained using GelCode Blue Stain (Part No. 24590). The black arrow points to the gel region where albumin is located on the duplicate gels before (A) and after (B) processing. |
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