Pierce 3x DYKDDDDK Peptide / 3x DYKDDDDK肽
货号：A36805,A36806
规格：5 mg,5 x 5 mg
价格：4591,13152
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| Pierce 3x DYKDDDDK 肽是一种具有以下序列的冻干肽：Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK)。其分子量为 2861.9 g/mol。使用时，在 PBS 中复溶至 1.5 mg/mL。该三聚体肽可用于从固定化抗-DYKDDDDK 亲和支持物中竞争性洗脱 DYKDDDDK 标签融合蛋白，特别是当 DYKDDDDK 标签蛋白对低 pH 值敏感时。请注意，Pierce 3x DYKDDDDK 肽可能会干扰蛋白测定试验和 280 nm 处的吸光度。然而，通过脱盐可以将肽段从洗脱液中去除（例如，使用 Zeba 离心脱盐柱，货号89891）。 |
| 数据： |
C- and N-terminal DYKDDDDK-tagged SUMO protein was expressed in E. coli and purified using Anti-DYKDDDDK Magnetic Agarose (A) or Anti-DYKDDDDK Affinity Resin (B), respectively. Tagged protein was competitively eluted with 3x DYKDDDDK peptide and analyzed by SDS-PAGE. Comparison of the starting lysate and elutions show effective capture and elution of DYKDDDDK-tagged protein. |
QQ:1749072012