3'-DMT-dG(iBu)-Suc-CPG; 1000 Å
货号:BG1-1200i-1
规格:1 g
价格:1055
产品类型:核酸合成
品牌:LGC Biosearch Technologies
| 3'-DMT-dG(iBu)-Suc-CPG; 1000 Å is an inverse linkage CPG used to create a 3'-3' linkage on the end of an oligonucleotide. An inverted 3'-3' linkage is desirable when needing to block polymerase extension through the 3'-terminus of a PCR probe; additionally, oligonucleotides with inverted 3'-linkages are used for some sequencing techniques. The 1000 Å pore size is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性: |
| - Chemical Name: 3'-DMT-dG(iBu)-Suc-CPG; 1000 Å- Appearance:White Powder- Extinction Coefficient at 260 nm: 12010 |
| 产品用途: |
| - Protecting group deblock conditions: 3'DMT groups detritylate slowly. Increase detritylation time by 50%.- Cleavage conditions: Use concentrated ammonia for 90 minutes at 25 °C when using standard amidites or 1:1 ammonia:methylamine (AMA) for 25 minutes at 25 °C.- Deprotection conditions: Use concentrated ammonia for 5 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 329.2 |
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