DABCYL-Suc-CPG (5'-DMT-mdC(TEG-DABCYL)-Suc-CPG); 1000 Å
货号:BG1-5025S-1,BG1-5025S-100
规格:1 g,100 mg
价格:11256,1407
产品类型:核酸合成
品牌:LGC Biosearch Technologies
Biosearch Technologies’ 5'-DMT-mdC(TEG-DABCYL)-Suc CPG is based on a modified cytosine residue linked to the Dabcyl chromophore via a triethyleneglycol spacer. The 3' end is conjugated to the CPG support via succinyl linkage. Our 5’-DMT-mdC(TEG-DABCYL)-Suc-CPG is specifically suited for the preparation of molecular beacons or fluorescent energy transfer probes. DABCYL is used as a quencher for fluorophore groups such as fluorescein, TAMRA and JOE. |
特性: |
- Appearance: orange powder- Absorption Maximum (Lambda Max):472- Extinction Coefficient at Lambda max:32000- Extinction Coefficient at 260 nm: 14333 |
产品用途: |
- Synthesis conditions: Follow the instrument manufacturer's protocol when using this product.- Cleavage conditions:Use concentrated ammonia for 90 minutes at 25°C.- Deprotection conditions:If using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC)) deprotect in concentrated NH4OH for 1 hour at 60°C. For standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated NH4OH for 5 hours at 60°C. If using base sensitive fluorophores (ie. TAMRA or Rhodamine based dyes) we recommend deprotecting oligonucleotide in t-butylamine/H2O (1:3) for 12 hours at 60°C- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:757.77 |
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