BHQ-0 CPG; Glycolate, 1000 Å
货号：BG1-5040G-1,BG1-5040G-100
规格：1 g,100 mg
价格：21105,2673
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-0 Glyc CPG is used to add the dark (non-fluorescent) quencher BHQ-0 at the 3' end of an oligonucleotide. BHQ-0 quencher has maximal absorption in the 430 to 520 nm range, which provides excellent quenching of fluorophores emitting in this range. Biosearch Technologies BHQ-0 Glycolate CPG has a glycolate linkage to the CPG support. The glycolate linker allows for rapid cleavage of the oligonucleotides and is labile enough for base sensitive oligonucleotides. The 1000 Å CPG is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| - Chemical Name: BHQ-0 CPG; Glycolate, 1000 Å- Appearance:orange powder- Absorption Maximum (Lambda Max):493- Extinction Coefficient at Lambda max:34000- Extinction Coefficient at 260 nm:7700 |
| 产品用途： |
| - Cleavage conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.- Deprotection conditions: When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 479.47 |
| 数据： |
| |
QQ:1749072012