BHQ-2 CPG; Glycolate, 1000 Å
货号：BG1-5042G-1,BG1-5042G-100
规格：1 g,100 mg
价格：21105,2673
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| BHQ®-2 Glycolate CPG is used to add the non-fluorescent quencher BHQ-2 to the 3' end of an oligonucleotide. BHQ-2 has maximal absorption in the 560 to 670 nm range, which provides excellent quenching of fluorophores emitting in this range, eg TAMRA, ROX, Texas Red® and Cy3™. BHQ-2 Glycolate CPG has a glycolate linkage to the CPG support. The glycolate linker allows for rapid cleavage of the oligonucleotides and is labile enough for base sensitive oligonucleotides. The 1000 Å CPG is best suited for the synthesis of DNA sequences up to 100-mers in length. |
| 特性： |
| - Chemical Name: BHQ-2 CPG; Glycolate, 1000 Å- Appearance:purple powder- Absorption Maximum (Lambda Max):579- Extinction Coefficient at Lambda max: 38000- Extinction Coefficient at 260 nm:8000 |
| 产品用途： |
| - Cleavage conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or
t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.- Deprotection conditions:When using fast deprotecting amidites (eg. C-Ac, G-DMF, G-PAC) deprotect in concentrated ammonia for 1 hour or AMA for 30 minutes at 60 °C. When using standard amidites (eg. C-Bz, G-iBu) deprotect in concentrated ammonia for 5 hours at 60 °C. If you are using base sensitive fluorophores (ie. TAMRA) we recommend deprotecting the oligonucleotide in t-butylamine/H2O (1:3) for 12-16 hours at 60 °C.- The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is: 556.46 |
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QQ:1749072012